Journal: Journal of Cancer Science & Therapy

Article Title: Silicate Granules Preconditioned with Human Bone Marrow Mononuclear Cells Improve Osteogenesis in Bone Sarcoma Patients

doi: 10.4172/1948-5956.1000370

Figure Lengend Snippet: Figure 2: A: Surface antigenic profile of BMMCs obtained from bone marrow aspirates at the first culture passage analyzed by FACS. a: BMMCs control. b-d: FACS setting with fluorochromes. e: CD34 and CD15 surface antigens co-expression characterization. f: 85.7% of BMMCs expressed CD90 antigen. g: 95.1% of cells expressed CD105. h: co-expression of CD90 and CD117 antigens. B: percentage of positive cells to other antigens such as CD14, and CD11b as indicated.

Article Snippet: The following markers were assayed: CD44 (clone#2F10 from R&D System), CD45 (Bioscience, clone #HI30), human Stro-1 (cat. 340106 BioLegend,), CD73 (Ecto50-nucleotidase, Biosciences clone #AD2), CD90 (Thy-1, clone #5E10), CD105 (Endoglin, Bioscience clone IgG1 SN6) CD29 (clone 4G7-2E3 form R&D system), CD11b (Mouse IgG2b Clone #238446), CD34 (Clone #756510) CD14 (Mouse IgG1 Clone #134620) from R&D systems and CD 235 (clone#HI264 from BioLegend).

Techniques: Control, Expressing

Journal: PLoS ONE

Article Title: In Vitro Characterization of Circulating Endothelial Progenitor Cells Isolated from Patients with Acute Coronary Syndrome

doi: 10.1371/journal.pone.0056377

Figure Lengend Snippet: Primary EPC/ECFC colonies were generated by plating patient PBMC in M 5100 medium, as detailed in the Method section. In A , after the colony identification (at day 5 after plating), medium was change (arrow) and replaced either with fresh M 5100 , or M EGM or M 199 and the development of the colonies was monitored over the time. The growth kinetics of a representative experiment out of five independent experiments is shown. At each indicated time point, the mean cell number/ECFC was determined by two independent operators; standard deviations were below 10% and are not shown. Asterisk, p<0.05. In B , immunocytochemical analysis of in vitro expanded EPC/ECFC documenting positivity for CD105 antigen (original magnification: 20X) and for the specific endothelial marker Factor VIII (original magnification: 40X). In C , FISH analysis performed on in vitro expanded EPC/ECFC by using the centromeric enumeration probe CEP9 (white arrows) documenting a normal diploid chromosomal pattern (original magnification: 40X).

Article Snippet: In order to analyze the immunophenotypic pattern of primary EPC/ECFC and CFU-EC, cells were detached with trypsin/EDTA (Gibco, BRL, UK) before the specific staining for flow cytometric analysis with the following Ab: CD45 Ab (2D1-APC), CD31 Ab (WM59-FITC), CD184 Ab (12G5-PE), CD105 Ab (SN6-PE), CD14 Ab (MΦP9-PE), CD146 Ab (P1H12-PE) (all purchased from BD Biosciences Pharmingen), CD34 Ab (QBend/10-PercP; Serotec Ltd., Oxford) and CD133 Ab (AC133-PE; Miltenyi).

Techniques: Generated, In Vitro, Marker

Journal: International Journal of Molecular Sciences

Article Title: Expression of Musashi-1 During Osteogenic Differentiation of Oral MSC: An In Vitro Study

doi: 10.3390/ijms20092171

Figure Lengend Snippet: Expression of mesenchymal stromal cell (MSC) surface markers.

Article Snippet: Anti-CD90 conjugated with fluorescein-5-isothiocyanate (FITC) (eBioscence, clone eBio5E10), anti-CD73 conjugated with phycoerythrin (PE) (BD Pharmingen, cloneAD2-PE) and anti-CD105 conjugated with allophycocyanin (APC) (Serotec, clone SN6) were studied for positivity; anti-CD14-FITC (BD, clone M5E2), anti-CD31-PE (R&D, clone 9G11), anti-CD34-PE (BD, clone 2D1) and anti-CD45-APC (Caltag, clone HI30) were evaluated for negativity.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Ectopic Bone Formation by Mesenchymal Stem Cells Derived from Human Term Placenta and the Decidua

doi: 10.1371/journal.pone.0141246

Figure Lengend Snippet: Antibodies used for characterizing CMSCs and DMSCs by flow cytometry.

Article Snippet: CD105 , APC , SN6 , 0.5μl , eBioscience.

Techniques: Cytometry

Journal: PLoS ONE

Article Title: The Cell Surface Proteome of Human Mesenchymal Stromal Cells

doi: 10.1371/journal.pone.0020399

Figure Lengend Snippet: Expression of CD markers on human mesenchymal stromal cells.

Article Snippet: CD105-APC (clone SN6) was purchased from Invitrogen.

Techniques: Expressing